Determination of Gardnerella vaginalis Genome Size by Pulsed-Field Gel Electrophoresis
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چکیده
منابع مشابه
Determination of Gardnerella vaginalis genome size by pulsed-field gel electrophoresis.
The chromosomal DNA of four strains of Gardnerella vaginalis were digested with rare cutting restriction enzymes and analyzed by pulsed-field gel electrophoresis (PFGE). The four strains studied were two clinical isolates (GVP 004 & GVP 007) and two American Type Culture Collection strains (ATCC 14018 & ATCC 14019). The restriction enzyme SfiI generated two DNA fragments of about 0.6 Mb and 1.1...
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Genome sizes of six different Wolbachia strains from insect and nematode hosts have been determined by pulsed-field gel electrophoresis of purified DNA both before and after digestion with rare-cutting restriction endonucleases. Enzymes SmaI, ApaI, AscI, and FseI cleaved the studied Wolbachia strains at a small number of sites and were used for the determination of the genome sizes of wMelPop, ...
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Pulsed field gel electrophoresis (PFGE) is a quick and reliable procedure to resolve DNA molecules larger than 30 kb by applying an electric field that periodically changes direction. This technique can be used to estimate genome size of a microorganism, to reveal if a genome is circular or linear, to indicate the presence of megaplasmids, and to show if a strain contains only one or more chrom...
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DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
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ژورنال
عنوان ژورنال: DNA Research
سال: 1994
ISSN: 1340-2838
DOI: 10.1093/dnares/1.3.115